5. 心臓発作予防（Protection Against Heart Attack Damage）
he effect of thymoquinone, an active component of Nigella sativa, on isoproterenol induced myocardial injury.
Myocardial injury constitutes a major cause of
morbidity and mortality in humans. Present study aimed to investigate
protective role of thymoquinone, which is an active principle of Nigella sativa
(N. sativa) seed (Commonly called as black seed), in isoproterenol induced
myocardial injury, a classical example of excess catecholamines related
coronary insufficiency and stress cardiomyopathy. Thymoquinone, in olive oil,
was administered orally (12.5, 25 and 50mg/kg) to three groups of Wistar albino
rats for 7 days, while two control groups were given plain olive oil.
Thereafter, thymoquinone receiving groups and one control group were injected,
subcutaneously, with isoproterenol (125mg/kg) for 2 days. Myocardial injury was
assessed by biochemical markers (plasma LDH, TBARS, GR & SOD and myocardial
GSH/GSSG ratio) and cardiac histopathology. Plasma LDH, TBARS and GR increased
in control groups receiving isoproterenol, while there was a dose related
decrease in these markers in thymoquinone treated groups, down to levels in
controls given olive oil only. Decrease in plasma SOD and myocardial GSH/GSSG
ratio and histological changes produced with isoproternol were also reversed in
thymoquinone treated rats. Results of our study revealed that thymoquinone
protects the heart from injury induced by isoproterenol.